Published Book Detail

Fundamentals of Microbiology – A Laboratory Manual

Saba Sana^1*, Ali Ahmad Sheikh², Zoya Maheen², Nadia Mukhtar², Tahir Yaqub², Sikander Ali¹, Muhammad Nouman Aftab³, Iqra Liaqat⁴

¹Department of Microbiology, Ikram Ul Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan

²Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

³Ikram Ul Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan

⁴Department of Pharmacology, University of Veterinary and Animal Sciences, Lahore, Pakistan

*For Correspondence

Sabasana22@gmail.com

Fundamentals of Microbiology – A Laboratory Manual is a guide for the students and laboratory personnel in the field of microbiology and focusing basic techniques of microbiology. This manual begins by introducing laboratory equipment and instruments with their working principle and use. It also focuses on different sterilization techniques for aseptic environment and their specific use in microbiology laboratory. Isolation and identification of microorganisms is an essential step in microbiology. This manual devotes a significant attention to cultivation techniques, culture media and microscopic study. Different staining techniques and micrometry are part of this laboratory manual as well. Various methods for microbial counts are mentioned along with some important bioassays such as antibiotic susceptibility testing. It combines theoretical knowledge and practical application of technique providing necessary tool to conduct experiments. Following this manual user will get a solid understanding of fundamental techniques in microbiology and develop competency to pursue advanced research.

Keywords

Microbiology, Sterilization, Inoculation Techniques, Micrometery, Microbial Counts, Antibiotic Susceptibility Testing